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GTP and RNA

throllen
in Biochemistry and Molecular Biology

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ok suppose i want to take a short DNA strand like this one 5-gatcctgcta-3 into RNA, and want it to be labeled. So radioactive GTP is used. And supposed if the GTP is radioactived with the additon of 4th phosphate group in the alpha postion, will the resulting RNA be radiolabeled? What about the beta position?

 

I don't get this at all :(

What?

 

How is it GTP if it has 4 phosphate groups?

 

You have to radiolabel the alpha phosphate on the nucleotide as this is the one that will be added to the RNA strand with the creation of a pyrophosphate.

 

You need to understand the mechanism of however a polynucleotide chain is created before you will understand this.

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