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ATP conundrum

Sinead
in Biochemistry and Molecular Biology

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Hi, Im new to this site, but from what I've read in the threads so far, there seems to be a wealth of useful information shared on the site!

I have a problem dispensing an ATP solution, which has confounded me, so any help would be gratefully appreciated!

 

Im dispensing an ATP solution through a nylon filter, but the first few ul that I dispense always has a lower concentration of ATP, then normalises. I thought that the ATP might be binding to the nylon, saturating the filter, but the same pattern of dispense happens for every plate. Any ideas at all??

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