IN order to investigate the action of bacterial membrane protein that is a light-driven proton pump, you purify protein and allow it to self-assemble together with phospholipids into liposomes that also contain an indicator dye,which is blue at high pH,colorless at neutral pH,and red at low pH.You then expose the liposomes , in an aquaeous solution, to sunlight.The interior of the liposomes stay colorless.Assuming that your bacterial protein has not been damaged by its purification and incorporation into liposomes, what it the most likely explanation for this results?

What's in the aqueous solution when you expose them to sunlight?

 

Nic

It doesnt say

"liposomes , in an aquaeous solution" means the liposomes are in water with nothing else.

 

This question is just testing your logic. The aim is to get you to recognise how to interpret observations made during practical tests, in the hope that you will develop a knack for experimental design.

 

You have been given the "specifications" for the bits and pieces in your test, so try to visualise the mechanisms that are at work.