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TLR-4, MyD88, TRAM, TRIF, TRAF-6, RIP-1, IRAK1-4 expression

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hello everyone. my problem is that with the help of what methods, experiments, I can detect the expression, activation of TLR-4 and other downstream proteins such as IRAK1, IRAK4, TRAF6, RIP1, and so on.

thanks in advance.

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