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Cytotoxicity Assay


KLIK

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Hello

I am working on cytotoxic T-cell assays. Iam planning to use dendritic cells pulsed with recombinant proteins as a stimulators in Cytotoxic T-cell assay (CTL assay). I have used particular cell lines (tumor cell lines) as stimulators in all the CTL assays that i have done so far in which i used to treat the tumor cells with mitomycin C before using them as stimulators. Can i follow the same procedure with pulsed DCs also. I have tried to find specific protocol for using pulsed DC s as stimulators but all the references i have gone through just mention this in few words but not any clear procedure. Can anybody help me with this or u can tell me references in which i can find the clear and complete procedure. I appreciate ur help very much.

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