Jump to content

Cytotoxicity Assay

KLIK
in Microbiology and Immunology

Featured Replies

Hello

I am working on cytotoxic T-cell assays. Iam planning to use dendritic cells pulsed with recombinant proteins as a stimulators in Cytotoxic T-cell assay (CTL assay). I have used particular cell lines (tumor cell lines) as stimulators in all the CTL assays that i have done so far in which i used to treat the tumor cells with mitomycin C before using them as stimulators. Can i follow the same procedure with pulsed DCs also. I have tried to find specific protocol for using pulsed DC s as stimulators but all the references i have gone through just mention this in few words but not any clear procedure. Can anybody help me with this or u can tell me references in which i can find the clear and complete procedure. I appreciate ur help very much.

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.