broe Posted November 15, 2005 Share Posted November 15, 2005 Im trying to measure different chemokines and Th1/Th2 cytokines in stimulated PBMC cultures. I know its optimal to remove supernatants after 24 hours for some cytokines and after 48 hours for others. However I can only remove supernatants at one time point, so I was wondering could anyone tell me which would be the best time point to do this in order to achieve maximum detection of all cytokines. Thanks Link to comment Share on other sites More sharing options...
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