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Specifications of plasmolysis in von Klercker's original protoplast isolation?

Methaneontitan
in Biochemistry and Molecular Biology

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The history of protoplast isolation says that the first isolation of protoplasts was done by von Klercker in 1892, by first plasmolysing and then slicing up plant tissues. But what were the specifications of the plasmolysis, e.g. salinity level and time in the solution?

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https://www.scienceforums.net/topic/134339-specifications-of-plasmolysis-in-von-klerckers-original-protoplast-isolation/
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