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Chelex 100

CZU
in Biochemistry and Molecular Biology

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Hi

I want to use Chelex 100 resin to extact DNA but in catalogue i found two kinds of this reagent (with dry mesh size 100-200 and 200-400). Do you know practical differences between these reagents?

The larger beads isolate smaller biomolecules and the smaller beads larger biomolecules. If you're just doing a PCR clean, I don't think it makes much difference.

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I want to extract bacterial DNA from various food matrix which have inhibitory influence on PCR reaction. In Biorad catalogue i have read that the 200-400 is designed for DNA extraction but 100-200 is more often found in references.

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