hilaryhurley Posted September 14, 2005 Share Posted September 14, 2005 can someone out there please donate a protocol for isolating human neutrophils from human blood. I did it about 8 years ago but cannot recall technique. involves dextran and ficoll but cannot obtain any detailed experimental protocol. stressed out. Link to comment Share on other sites More sharing options...
zyncod Posted September 16, 2005 Share Posted September 16, 2005 I did it about a year ago. I think it was ficoll/hypaque. Fairly simple, but MACs would probably work better. Link to comment Share on other sites More sharing options...
LucidDreamer Posted September 16, 2005 Share Posted September 16, 2005 " J. E. Coligan et al., Eds., Current Protocols in Immunology (Wiley, 1999)." If you have access to a library or online library. Link to comment Share on other sites More sharing options...
sasu Posted October 13, 2005 Share Posted October 13, 2005 can anybody tell me process for breaking the neutrophils:mad: Link to comment Share on other sites More sharing options...
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