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neutrophil isolation protocol

hilaryhurley
in Biochemistry and Molecular Biology

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can someone out there please donate a protocol for isolating human neutrophils from human blood. I did it about 8 years ago but cannot recall technique. involves dextran and ficoll but cannot obtain any detailed experimental protocol. stressed out. :eek:

I did it about a year ago. I think it was ficoll/hypaque. Fairly simple, but MACs would probably work better.

" J. E. Coligan et al., Eds., Current Protocols in Immunology (Wiley, 1999)."

If you have access to a library or online library.

  • 4 weeks later...

can anybody tell me process for breaking the neutrophils:mad:

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