Does it need control media and sterility test for bacterial inhibition testing?

[Vianeye Han]

I tested the inhibitory power of the bacterium Pseudomonas aeruginosa, with the well diffusion method, I used nutrient agar for growth media and Mueller Hinton Agar as a inhibitory test medium, for the positive control I used the antibiotic ciprofloxacin and the negative control was ethanol because I used ethanol extract from Moringa leaves. Are control media and sterility tests required as well? 

Thanks in advance 🙏🏻