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genetics experiment design


Alexgrahama98

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I am given 3 traits

Trait 1, wing shape, two different alleles Dumpy and Scalloped;

Trait 2, tarsi, two different alleles Truncated and Wild Type;

Trait 3, two different alleles Vestigial and Wild Type;

 

Note: These traits are imaginary and bear no relationship with the real

ones in terms of inheritance pattern or chromosomal location.

A first cross is set up with a Scalloped, Wild Type, Vestigial male and a

Dumpy, Truncated, Wild Type female. On the F1 we obtain 132 male flies

with the following phenotype: Dumpy, Truncated, Wild Type and 127

female flies with the following one: Dumpy, , Wild Type, Wild Type.

A second cross is set up with a female from the F1 (Dumpy, Wild Type,

Wild Type) and a male with the following genotype: Scalloped, Truncated,

Vestigial.

Working from the progeny of the cross I think I have deduced that trait 1 and 2 are linked on the same chromosome with and Rf of 15%. trait 2 is X linked (I think) if anyone wants to check the workings i'll send a photo of the progeny separated according to phenotype and gender summarised in a table. My task now is to design an experiment to identify the genotype of the P1 parents and I have a couple of problems. One is I'm not sure what to start with, do I assume that I only have the F2 progeny to work with and that the F1 and P1 flies are all dead? Id love someone to point me in the right direction with regards to what kind of genetic tools will be needed.

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