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Biotinylated SSR Probe Help

michael_ohara
in Biochemistry and Molecular Biology

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Hello!

I'm trying to probe microsatellites (SSRs) in a mantid (T. sinensis) using two biotinylated oligo sequences [(CA)10 and (GA)10].  I know the concentration the probes need to be, but I don't know the volume.  I've looked through numerous papers and can't find anyone who's reported the volume used.  I'm going to digest 1ug of genomic DNA with RsaI, hybridize the probes, then pull them out with streptavidin magnetic beads.  There's about 5700 Mbp in a diploid cell if that helps.  Any help would be greatly appreciated.  Thank you!

Mike

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