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Can you use segregation analysis to determine foreign gene number in plants?

Dean22April
in Genetics

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If I have a wheat plant expressing a transgene but I'm unsure of the copy number of that transgene (e.g. using particle bombardment you often get the plasmid DNA inserted into the genome more than once), could I use segregation analysis to determine how many times the transgene has gone into the plant?

I.e. if I had the foreign gene inserted just once would I get 15: 1 ratio in F2? How would this change if I had more than one foreign gene? Would this method even work? Any other method to determine how many times my transgene had gone into wheat?

Thanks for any help :)

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