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FStat and genetic data help

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Hello I am approaching with genetic analysis and FStat and I have a lot of doubts.

If I have a sample composed by a single population (so i don'thave a subdivision in subpopulations) why cannot I calculate deviation from Hardy-Weinberg equilibrium with FStat?

And when I have more than one population which parameters should I choose from the FStat window? And how could I read the output file?

I am really confused and I appreciate any suggestion! Thanks

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