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MALDI-TOF analysis

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Hello everyone! I'm a new member and this is my first time posting here for help.

 

I have an assignment where I have been given MALDI-TOF data for an apomyoglobin standard and a thrombin digested GST tagged of an unknown protein. I am having trouble identifying the peak indicated with a question mark in the standard data. So far, I have identified the middle peak in the thrombin digested protein as GST, while the first peak (11499.1) as the unknown, which I would be analysing with bioinformatic methods. I would really appreciate it if someone could help me or rather give me information as to how to identify the remaining peaks in both datas. THANK YOU for reading!

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  • 11 months later...

hello, did you ever find an answer to this? what is the question mark labeled peak?

image.png.f6408310479464eb2abdceb50a961281.png Lest consider it without the middle right Heme, your data are 100% sure?

I took a look, and it said that technology break the large into not so small part ? I need more Info? 

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