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Help with transcription mediated DNA looping

lezinal
in Genetics

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You have a DNA template containing a p53 binding site that is 1 kilobasepair upstream of the core promoter. You also have the following purified proteins: p53 m,ediator, TFIIB, TFIID, and RNA Polymerase II. Feel free to clone/express/purify modified

versions of the proteins or additional protein reagents if necessary. I would like to design an in vitro experiment (i.e. not in cells) that will determine the frequency and duration (i.e. dynamics) of transcription factor mediated looping of the DNA template. How can I set this up and what should I use as positive and negative controls. Thanks

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