mohamed samir
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I usually got an melting curve with multipeak. Can anyone tell me what is the exact cause for this
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Hi everybody. Iam doing isolation of miRNA from tracheal tissue of infected mice. What is bothering me really that I got surprised when I end up with low RNA yield of the sample. I red the protocol carefully and found nothing wrong with my work. Have any one knowledge what is the difference between chlroform and the chloroform /isoamyl alcohol mix. I mean the protocol stated that you have to be sure that the chloroform is not mixed with iso amyl alcohol. But I red that the iso amyl alcohol reduces the RNAase so i suppose that it will be beneficial for the isolation.
I would appreciate it if any one help me.
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First of all, Iam happy that you do your best to capture some valuable information from the book in your hand.
Here and as far as I know that the surface of the culture bottle or the culture petri dishes characterized by presence of material, polystyrene. that help the cell to adhere to the surface and I think also when u provide the cell with nutrient and medium , after 1-2 hours it will start gowing and adhere to the surface
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Co- culture experiment
in Biochemistry and Molecular Biology
Posted
Alittle bit stricky for me to ask question like this.
Iam going to perform an experiment on a co- culture consists of both human A549 pneumocyte II cells and murine macrophage J774 cell line to test the effect of miRNAs on influenza virus replication .
I have no protocol for this and iam confused really whether I will be able to blend two cell line from different host. However, the media in both cells are differ , is this will pe appropriate medium for both cells ?
Should I used human macrophage cell line to be compatable with the A549 cell as it is from human ?
Dpepnding on which criteria, will our biculture succeed ?
Can I culture two differenrt cell line ( for example, A549 and J774 ) at the time both of them was cultured in different medium ?
Can I culture two differenrt cell line ( for example, A549 and J774 ) that isolated from two different animal host or human ?
Thanks