MicroGirl

Thanks very much! Its probably alright then

Thanks very much for your responses! Greatly appreciated

Hi there,

I need ampicillin solution at 100mg/ ul I was wondering how i go about making this up? How many Grams per ml.

Thanks heaps!

Hi everyone,

I made ampicillin-LB media on Wednesday and poured it into plates on wednesday afternoon. After allowing them to set i turned the plates over and left them on my desk wednesday night, Thursday day and Thursday night:doh:. This morning (Friday) i have packed them and put them in the fridge.

I was just wondering, was it a mistake to have left them a whole day at room temperature? Has my media or the ampicillin gone off over this time? Can i still use my plates or should i just make new ones?

Thanks heaps for your help,

Christy.

Thanks heaps!

HI,

I was just wondering if it was ok to store your PCR in -4C overnight and thawing to do a PCR clean up the next day?

Will this screw up my results or should it be ok to do

THanks,

Christy.

HI,

I was just wondering if it was ok to store your PCR in -4C overnight and thawing to do a PCR clean up the next day?

Will this screw up my results or should it be ok to do

THanks,

Christy.

I have a 5uM Concentration of primer stock.

I need to find out how many nanograms/Microliter i have in this stock soltion.

Any help appreciated! thanks

Hi im an honours student trying to pull out a clone library.

I have a question conserning the colour of X-gal.

I was required to make a solution of X-gal and it is clear in colour. When i compare the colouring of my x-gal to that of my supervisors, i can see that his is yellow in colour. Does this difference mean anything? Could it cause any differences wen spreading it on an Ampicillan-LB plate for the growth of clone colonies? Why is mine not yellow?

Any help appreciated

THank you!!

Thank you everyone for your responces, its nice to know that people out there are willing to give support. When i mean 'fail' I mean my experiment didnt work...not a 'negative' result but wasnt sucessfull. For example im trying to build a clone library. its the first time ive ever done one. I did the ligation and transformation and plated the cells out only to find very few colonies growning, not enough for me to build a clone library from. Also i just make silly mistakes, wich i know i should'nt get too upset over...but i think its because i care and try so hard that i end up making these mistakes. (like over diluting my oglionucliotide primers by 100 times by using the wrong measurment on a pippett). They are mistakes i KNOW not to do but i do them. Its not that im careless....Because i care and worry so much. My supervisor says that i stress too much. So i guess i have to try and relax a bit.

What im really worried about is getting some results to talk about in my thesis. As an Honurs student i only have 1 year to complete a project and talk about it. I feel that i have been given quite a challenging project to complete in 1 year...mostly due to my inexperience in the lab.

Thank you all for your replies keep them comming!

Hi everyone ,

Ive come to the forum looking for some encouragement from all my fellow scientists. Im a university Microbiology honours student that has been having a few troubles with her project. Every time i try to do an experiment, it fails and i have to do it again and again to get it to work. I tend to make allot of stupid mistakes and ask allot of stupid questions and at the moment i feel like a real idiot . Im really afraid that i wont get my project done in time for me to write my thesis as i am hoping to get a 1st class honours so that i can apply for a PhD scholarship.

I was just looking for some words of encouragement and/or advice to help me get through this difficult year.