tman_ap

So, I am suppose to cut the plasmid and the insert with the SexAI enyme.. then join the insert I cut parts together and insert THAT insert into the area I cut the plasmid and then connect the other parts of the plasmid with the insert in between?

If not, please explain more. I am so confused at this moment!

It's a genetics assignment.

So, after I cut off the site from the SexAI, I just attach the rest of the sequence (after the ligase) together?

If yes... do I then attach the plasmid and the insert together with each other to form huge sequence?

Question:

You are going to clone a part of this small DNA insert into a plasmid by cutting out the insert with SexAI restriction enzyme, opening up the plasmid with SexAI, and then ligating the two pieces together. Given the sequances of the insert and part of the plasmid (below), show where the SexAI sites are in both sequances and then write the sequence of the part of the plasmid including the insert once the pieces have been ligated. Indicate the insert, the plasmid, and the SexAI sites on the new sequance.

Insert: 5' CGATACCTGGTGATTGTAAGCCACC... 3'

Plasmind: 5' GTAATGCCATTCACCTGGTTTAGGC... 3'

What I know:

I know that the SexAI enzyme cuts at:

5'ACCWGGT 3'

3'TGGWCCA 5'

However, W is replaced with T 5-3' and with A from 3-5' here!!!

5'ACCTGGT 5'

3'TGGACCA 3'

It cuts as fallowing:

Insert: 5' CGATA || CCTGGTGATTGTAAGCCA || CCTGGTTAACG 3'

Plasmind: 5' GTAATGCCATTCA || CCTGGTTTAGGCATGATCT

NOW??

I am stuck here and I don't know what else to do. Please Help!!!

The primary function of hemoglobin is to carry oxygen from the lungs to tissues. The Bohr Effect aids hemoglobin to carry oxygen from the lungs to tissues by releasing oxygen to the tissues when the concentration of hydrogen ions becomes large. The Bohr Effect allows the blood to unload oxygen for use by the muscles at low pH. Therefore, the Bohr Effect is necessary for the body to function.

Yes... I was looking at the Oxygen Dissociation Curve to see if I can come up with an answer... but I don't understand the curve too well and I guess that is what I am stuck!

In humans, the urge to breathe result from high concentration of O2 in the blood; there are no direct physiological sensors of blood pO2. Skindivers often hyperventilate (breathe rapidly and deeply for several minutes) just before making a dive in the belief that this will increase the O2 content in their blood.

a. Does it do so?

b. Use your knowledge of hemoglobin function and the Bohr effect to evaluate whether this practis is useful.

Are the large number of microorganisms found in the mouth a cause for concern? Explain

Why is a blood agar, rather than a nutrient agar, plate used for the culture from the mouth?

Does anyone know what the source of blood in the hepatic portal system is?

I know that most arteries in the adult body carry oxygen-rich blood, and the veins carry oxygen-depleted, carbon dioxide-rich blood. But how does differ in the pulmonary arteries and veins?

Trace (describe) the pathway of a carbon dioxide gas molecule in the blood from the inferior vena cava until it leaves the bloodstream. Also, name all structure (vessels, heart chambers, and others) passed through en route.

I need help with this please!!!