Ninja2188

Okay, so I've noticed that when I make TSA, and after I've sterilized, and after storage at 60 degrees Celsius, that there is this white stuff that forms after it's been sitting for a day or so. And I plated the TSA this time to see if there would be any growth, and there was. Does anyone have a suggestion as to why? I've sterilized it before storing it, so why would there be any growth?

i could probably inoculate every couple of days. I've got some going on a TSA slant to see how that will go. Just to test things out.

I would need to sustain it for a week.

Trying to make do with what I have here in the lab...can K. pneumoniae grow and be sustained in a litmus milk? Or is that more just for testing. I don't have the exact agars to grow it on and am just trying to see what I can grow it on with the things I do have. We have to use it for a capsule staining lab, and then we'll need it again for gram staining next week. So just wondering the best way to preserve it until next week.

Hi all!

I'm a lab tech for a microbiology lab, and it's been a little while since I've done microbio. So just a quick question I had, when you autoclave TSB in the test tubes, do you put a cap on it? or no? Just wondering if one way works better than the other. Thanks!