jdashcup

I am trying to get my protein into a deuterated buffer for NMR. I tried using the Millipore centrifugal filtration units and lost most of my sample to it. I am thinking the protein is sticking to the membrane. I am looking for a substitute buffer exchange method. Dialysis isn't an option because of the cost and quantity of solvents required. Any suggestions would be helpful.

Thanks.

CUP