cazmantis

That's great - thanks so much for your help. Making a lot more sense now. Best, Caroline

Hiya! Wow thanks so much for the replies - very useful stuff so far and has served to illuminate my own lack of knowledge of the subject! I think I am getting a little confused with these things. The PREDICTED sequences I am looking at are nucleotide sequences and I am not sure how I would cross reference that against a genome. It just doesn't seem to make sense to me so I assume that my lack of experience in the field means I don't have access to all the facts! For example NCBI accession number XM_001120951 - it says this nucleotide sequence has been predicted from the genomic sequence. I am finding this quite confusing as surely the nucleotide exists or it doesn't. I want to understand how these predicted sequences are different to (let's say) "normal" sequences. I understand this may be a little in depth to expect an answer on but if anyone could perhaps reccomend a book which may cover this aspect of genomes that would be just as helpful for me. Thanks so much for your help, Caroline

Bit new to bioinformatics and just wondered how useful PREDICTED amino acid sequences derived from the genome are (I have found several in NCBI). If trying to BLAST these sequences to find conserved sequences, are the results going to be of use or is it better to not bother with these sequences at all? Thanks, Caz