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bren2010

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  1. bren2010 started following Quantitative PCR v Semi-quantitative PCR
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    What are the effects of zinc sulfate, NaCl and sugar on PCR

    bren2010 posted a topic in Biochemistry and Molecular Biology
    Hi I wish to isolate eggs from sheep faeces and subsequently extract DNA and run a PCR from them. Part of the egg isolation technique involves floating them on a dense liquid. The three commonly used floatation liquids are Zinc sulfate, saturated sugar and saturated salt solutions. I was wondering what are the effects of these substances on the performance of the PCR reactions ?

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