cristianammg

Lipases are enzymes - proteins essentially.

 

As far as I know, a change in pH can change the solubility of proteins a lot. If they are soluble, you might convert it into insoluble, and use a filter.

 

Alternatively, you might try a real extraction, with a solvent (any water-insoluble solvent might work). But in a biological medium, any solvent will extract a lot more than just your enzyme... and it might kill your microorganisms.

 

Can't you somehow immobilize the enzymes while they're still in your medium? I know there are tricks to immobilize enzymes, but I am not familiar with the techniques.

 

p.s. (1) I'm a chemical engineer - therefore only half-qualified to give you advise.

p.s. (2) have a look in our other sub-forums too. We also have a Biochemistry and Molecular Biology forum that might interest you.

Ok... thank you very much... I'll search in other areas and I'll take your tips in consideration

Hello everyone (This is my first post here)

I'm a PhD student (More Info) and I need a method to extract the produced lipase from an effluent. This lipase is from Y. lipolytica or C. cylindracea. I found lots of methods on the Internet and articles/papers but I want something really simple/rudimentary that doesn’t significantly modify the medium were lipase was produced.

Could you please help me?

Thank you all…