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hsiang

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    Is the copy number of combined vectors equal in E. coli cells?

    hsiang posted a topic in Microbiology and Immunology
    I have a series of combination plasmids based on the pET-28a vector, each of which contains the ppk gene from different bacteria inserted at the same site. The sequence length of these plasmids is approximately 7300 base pairs each. I am curious about whether the copy numbers of these plasmids remain consistent after they are separately introduced into E. coli cells?

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