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Molbio_learning

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  1. Molbio_learning

    Question on primer design

    Molbio_learning replied to Molbio_learning's topic in Biochemistry and Molecular Biology
    Thank you for the reply. Yes, I want to get biotinylated product directly during synthesis, how do I order such primer?
  2. Molbio_learning started following Question on primer design
  3. Molbio_learning

    Question on primer design

    Molbio_learning posted a topic in Biochemistry and Molecular Biology
    Hello everyone, this is my first post and I am hoping it is the right place to ask. I just recently started cloning, PCR design and similar. I have very basic question and would like to get an answer step by step if possible, using an example 🙏🏻 So I have a pool of oligos (with short stretch of the same sequence at 5’ and 3’ so I can do PCR with it, for example to introduce nuclease sites). My question is, how do I design a primer for PCR to introduce biotin site and let’s say one of any restriction enzymes?? I hope I provided enough of info, I need only one primer. thanks a lot!!! 🤓

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