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Posts posted by Summerdreams
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I really need to be specific with the amount needed for my school's curriculum. I also was going to use HCL or NAOH but I'm worried that I would use too much and kill the algae. Also, I want to use the buffer solution so that it would stay at a specfic pH for the duration of my experiment. I can call the seller of the growth medium and finds its ingredients but I won't know until tomorrow. Lastly, do you think I could use the Henderson Hasselbach equation to answer my question or would I need another equation? Thanks.
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My highschool science experiment is testing to see if there are differences in growth of algae if they are grown in growth mediums with different pH levels. The problem is that I am very confused on how to to calculate how many mL of phosphate buffer solutions I should use to change the growth medium to the desired pH. For example,
How many mL of a .1 M phosphate buffer solution (pH 5) should I add to 15 mL of a growth medium (pH 7.8) to lower the growth medium's pH to the desired pH level of 5? I believe that I need the Henderson Hasselbalch but I am very confused with the math. Any help would be much appreciated. If math is shown then I will be able to calculate the mL of buffer solution needed for my other levels of pH.
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How to measure growth of algae with a spectrometer?
in Biology
Posted
For a science experiment I am trying to measure the growth of algae grown in these test tubes. I have a spectrometer available and I have been told I can measure the growth of algae by using a spectrometer to help analyze the algae's density. However, I am unsure about the steps and what nm setting I should use. Can somebody please help?