lothloran
-
Posts
3 -
Joined
-
Last visited
Content Type
Profiles
Forums
Events
Posts posted by lothloran
-
-
How would it help?
In the economic sense of the word, perhaps.
I see how you've achieved nearly 8000 posts.
Do you have an answer to the question or are you just going to continue being pedantic?
-1 -
Hello forum.
I've recently decided to store my genetic material in case anything should happen to me, the problem is I do not have the money to store it in a sperm bank, but I have ready access to liquid nitrogen and can whip up ethyene glycol fairly easily as a cryoprotectant, The process itself is fairly straightforward and I'm confident that I can achieve effective results with a bit of trial and error.
My question is, when it comes to the dethawing process, I understand I need to wash out the cryoprotectant with a culture medium then separating the culture / glycol with a centrifuge. I'm not sure which culture medium would be the safest to work with and I am not sure whether a 4000-5000 RPM centrifuge would be effective or if I need something a bit more powerful in the 10000 RPM range.This is a serious post. Liquid nitrogen is 50 cents / L and paying to store my sperm is in the thousands. It seems like the rational thing to do.
0
First post - Cryogenic preservation questions
in Projects
Posted · Edited by lothloran
The purpose of the project is to preserve my genetic material and not have to pay thousands of dollars over the years to a sperm bank.
I have all the necessary equipment, the LN2 and C2H6O2, the dewar, and access to as much liquid nitrogen as I would ever need.