spectrophotometry for biomass quantification in Biochemistry and Molecular Biology Posted September 30, 2015 Hello, For my current thesis research I need to quantify the biomass of a bioreactor 5 x each day, therefor i use as a quick method a spectrophotometer. Now to make a standard curve, i dried a certain amount of biomass. The problem starts with the dilution: when i dilute this biomass it will not give a homogenous solution. Can I use sonification to make this homogenous? I know the cells will be broken but we still have all the biomass? Is this correct? 0
spectrophotometry for biomass quantification
in Biochemistry and Molecular Biology
Posted
Hello,
For my current thesis research I need to quantify the biomass of a bioreactor 5 x each day,
therefor i use as a quick method a spectrophotometer.
Now to make a standard curve, i dried a certain amount of biomass.
The problem starts with the dilution: when i dilute this biomass it will not give a homogenous solution.
Can I use sonification to make this homogenous?
I know the cells will be broken but we still have all the biomass?
Is this correct?