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SamP

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    Protein purification

    in Homework Help

    Posted · Edited by SamP

    So I have a "hypothetical" worksheet for biochemistry. I have to separate the following 8 proteins as much as possible:

     

    Bovine Serum Albumin

    Myoglobin

    Hemoglobin

    Cytochrome C

    Alpha-lactalbumin

    Ribonuclease

    Ferritin

    Catalase

     

     

    I started with Size-exclusion and got these for the light proteins:

    Cytochrome C

    M`yoglobin

    Alpha-lactalbumin

    Ribonuclease

     

    And heavy proteins:

    Hemoglobin

    BSA

    Ferritin

    Catalase

    I figured out ways to separate the light proteins further and essentially have them completely separated. What are some techniques I can use to separate the heavy proteins?
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