I would like to resolve a protein with a MW of 3 kDA by using a Tricine SDS page (16%), I've already got the standard (1,7 - 40 kDA) but every publications i found tell me that this kind of low range standard
are stained with a Coomassie blue G-250.
Is there any possibility to use a silver staining and still see the lowest range band ( 1,7 kDA ) or do i have to use a Coomassie to stain it ?
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Tricine SDS page
in Biochemistry and Molecular Biology
Posted
Hey everyone !
I would like to resolve a protein with a MW of 3 kDA by using a Tricine SDS page (16%), I've already got the standard (1,7 - 40 kDA) but every publications i found tell me that this kind of low range standard
are stained with a Coomassie blue G-250.
Is there any possibility to use a silver staining and still see the lowest range band ( 1,7 kDA ) or do i have to use a Coomassie to stain it ?
Cheers !