I'm a Master's student currently working in a lab which has been using vectors encoding shRNAs for transient knockdown in C2C12 cells. We're considering creating new vectors encoding AgoshRNAs to increase knockdown efficiency/ noise reduction, but since the science behind these hairpins is fairly recent, I was looking to get some insight from anyone who's used them - are there any problems associated with their use, and do they function as well as they should? I'd appreciate any advice!
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Anyone with experience using AgoshRNAs?
in Biochemistry and Molecular Biology
Posted
Hi all,
I'm a Master's student currently working in a lab which has been using vectors encoding shRNAs for transient knockdown in C2C12 cells. We're considering creating new vectors encoding AgoshRNAs to increase knockdown efficiency/ noise reduction, but since the science behind these hairpins is fairly recent, I was looking to get some insight from anyone who's used them - are there any problems associated with their use, and do they function as well as they should? I'd appreciate any advice!