Robertmg
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Hello everyone,
I just started as a research intern at a lab (at the undergraduate level), and I am reviewing a protocol I will be implementing, and I was just wondering what the term "batch bind" means. Does it have to do with allowing one substance to bind to another in a "batch" at a specified temperature?
Thanks for your help,
Robert
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Ways to Cleave FCP1 from GST Protein Tag
in Biochemistry and Molecular Biology
Posted · Edited by Robertmg
Hello everyone,
In our lab we have been attempting to cleave FCP1 protein from its GST tag by using TEV protease. So far, several batches of TEV protease have been tried, but we have not gotten a significant amount of FCP1. I was just wondering if anyone had any suggestions or ideas for alternative methods for cleaving FCP1 from its GST tag that have produced good results. Thanks for any ideas.