cells
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We have modified the current DNA cloning method. Our modified method is especially suitable for the challenging cloning (e.g., blunt-end DNA cloning, single-digested DNA cloning and some challenging double-digested DNA cloning). The modified method is simple and convient. We think it worth a try if you have some trouble during molecular cloning.
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If it is helpful, please do not forget to cite it in your publications, thank you before hand. Good luck for your work!!!
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new method to improve cloning efficiency
in Biochemistry and Molecular Biology
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this is a very short paper