Edwin_K

Hi all,

So i have this 2.5L of H2O2 (30%) stock, and i know the M of H2O2 in the stock is 22.05M,

How do i make 1M of H2O2 using a phosphate buffer solution (PBS)?

Thank you in advance!

Thanks studiot for replying, to clarify things:

Since PBS is used for substance dilution, i assume there will be no reaction between PBS and H2O2?

We were instructed to dilute PBS by a factor of 10 with distiled H2O, and then the diluted PBS was then diluted again with DD H2O by factor of 100, so can we call the solution A as PBS diluted by a factor of 1000?

We were then instructed to mixed the PBS (diluted by a factor of 1000) with pure H2O2 (30%) by the ratio of:

1ml H2O2 : 99ml PBS (diluted by a factor of 1000)

which creates solution B (diluted H2O2).
At this point, is it safe to say the concentration of H2O2 is 1%? Or can we calculate the molarity of H2O2 at this point?

Next, say 1.5ml of Solution B (diluted H2O2) was then mixed with 1.5ml PBS (diluted by a factor of 1000)..is there a way to calculate concentration of H2O2 in mol or other unit at this point?

Hello all,

I'm a physiologist by trade but was recently assigned a chemical job, and my chemist supervisor is not willing to help..would really need help from anyone right now.

- 1st, we mixed 1ml PBS solution into 9ml DD H2O and then 10ml of this solution was further mixed into 990ml DD H2O. (This final solution of PBS-DD H2O is hereby known as solution A)

- Say we mixed 1ml of H2O2 (30%) with 99ml of the solution A creating solution B,

- And then 1.5ml of solution B was mixed into 1.5ml of solution A, what is the concentration of H2O2 in the 3ml solution?

Thank you very much in advance!!

And sorry if im confusing and/or using inappropriate terms, im really not a chemist by trade.