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Susheel K Sharma

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    Biological Science, Plant Virology

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  1. Susheel K Sharma
    Hi I have a problem in the expression of plant viral gene in E. coli system. The gene is cloned in pET28a and the sequence is in frame (confirmed by sequencing). I have tried the expreesion in DE3 (BL21), PlysS, Rosette PlysS, but could not get the expression. The expected sixe of protein is 48 KDa and 14 KDa. Kindly suggest me some troubleshoot.
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