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Hi all, I am currently working on the MOA for a novel checkpoint inhibitor that is similar to PD-1. I already have a CMV assay showing nice blocking but what I would like to do is develop a DC/macrophage assay. Any ideas?

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Hi there, I was just wondering if anyone can provide me with some insight here. I am a novice molecular biologist and I am involved at looking at splice variants (alternative forms of the same gene) I am able to find mRNA for these variants but when I go to look at the protein (using an antibody that detects a region that is common in all the variants) I can't see them. I tried different percentage gels (for western) etc. Is it because the message isnt translating or something about the way the protein folds? Can anybody help me regarding proteins?