lezinal

It was homework but I was hoping to get other peoples thoughts. That's how you learn, not just by submitting and forgetting about it.

A female astronaut from earth was on a mission to Jupiter. There, she met a charming alien man. The two hit it off and decided to build a house in one of the twelve moons of Jupiter and start a family. Just like us, the aliens of Jupiter have 46 chromosomes and identical DNA sequence and epigenetic mechanisms of gene expression. However, they completely look different than us. They have giant ears and are twice our size. Their gestation period is 12 months instead of 9 months (it is part of the biology of their placenta and has nothing to do with the environment or size of babies). The astronaut and her alien mate welcomed a baby girl into their family. Interestingly, the gestation period of this baby was 12 months (despite developing in a human body). Moreover, she looked nothing like her alien farther and rather looked more like her human mother (normal human ears and regular human baby body size). Propose epigenetic mechanisms to explain these observations

You have a DNA template containing a p53 binding site that is 1 kilobasepair upstream of the core promoter. You also have the following purified proteins: p53 m,ediator, TFIIB, TFIID, and RNA Polymerase II. Feel free to clone/express/purify modified versions of the proteins or additional protein reagents if necessary. I would like to design an in vitro experiment (i.e. not in cells) that will determine the frequency and duration (i.e. dynamics) of transcription factor mediated looping of the DNA template. How can I set this up and what should I use as positive and negative controls. Thanks