allaroundanonymous

Does the type of [ultra]centrifuge you use matter? Basically, when trying to use the same protocol using a regular centrifuge, I got very loose and slanted "pellets" which were all but impossible to separate from the supernatant, even after centrifuging longer. I used RCF*time equivalent values to start with. Could it be that a similar protocol just can't be used with a regular centrifuge, or am I doing something wrong?