green15b

Dear All, I am trying to preverve Vibrio sp. in liquid formulation (in vegetative form). This is a salt tolerant vibrio and I am preserving the vibrio in 2% NaCl containing water. The preservation is done by diluting the culture broth (could not talk about exact composition - but it contain a mix of easy and difficult to ferment sugars) in the ratio of 1:5 in 2% NaCl water. This is a aerobic bacteria, but after preservation, due to closed environment there is now room for air exchange. I could see that the formulation keeps the viability of culture for 2 weeks but loosing the viability after that. Any suggestions about process improvement or suggestions about completely new process is most welcome. Thank you all for your kind help. Regards, Kumar