Ploab

Hi! I have a problem with a fused protein. I want to know the optimal pH and temperature range (among other physical properties) of a fused protein, made of two mini-proteins. However, I'm still waiting for my DNA synthesis to arrive in order to produce it and I only have a few properties of the mini-proteins. I do have my fused protein modelling and structure, but I cannot predict any of these parameters. Does anyone know about how can I predict (in the most accurate possible way) pH, temperature and isoelectric point of my fused protein without experimenting? Thank you!