stbell

I am attempting to prepare a spore suspension of Bacillus subtilis but am not having any luck geting rid of the vegetative cells... I have heat shocked at 70C for 15 minutes and centrifuged that heat shocked suspension at 5000rpm for 10 minutes twice in attempts to get rid of the vegetative cells but upon the confirmatory spore stain, several rods are still present (nearly as many as the control stain which was taken from the pre-heat shocked suspension). I have searched high and low for a little more guidance as how one separated vegetative cells from spores but haven't come across anything yet... any help would be greatly appreciated!