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ahmed2015

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    molecular biology

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  1. Please help me answer this question: You are provided with the following information: (i) the sequence of a cDNA fragment of the gene encoding a cell-surface plasmodium receptor. (ii) an expression vector containing T7 promoter and multiple cloning site including EcoRI and BamH restriction sites. a. Describe how you would clone the coding region fo the gene into the expression vector provided.
  2. You are provided with the following information: (i) the sequence of a cDNA fragment of the gene encoding a cell-surface plasmodium receptor. (ii) an expression vector containing T7 promoter and multiple cloning site including EcoRI and BamH restriction sites. a. Describe how you would clone the coding region fo the gene into the expression vector provided.
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