RonSean

I am currently trying to express a toxic Fab protein using e.coli. Unfortunately, I have to use this expression vector. I can successfully make the Fab protein at the 2ml scale but have not been able to express at larger 50-100ml scale. Details: pComb vector, SB media, Carbenicillan, with and without glucose, IPTG expression system (non T7 driven) and an e.coli strain that has the laq Iq gene to over express the lac repressor. I am a molecular biologist and am new to protein expression in e.coli. Any advice would be greatly appreciated.