grrompala Posted March 17, 2016 Share Posted March 17, 2016 (edited) Hi, I'm using the Qiagen miscript rtqPCR kit for small RNA quantification. I did a gel purification for RNA bands >40bp and those <40bp. When I then performed miscript with miRNA specific primers, I still had a strong signal ~24 ct for the >40bp gel fraction. However, melt curves and pcr products looked identical between >40bp and <40bp. Could it just be my gel run wasn't efficient in separating the bands? Seems hard to believe. Any insight would be helpful, thanks! Edited March 17, 2016 by grrompala Link to comment Share on other sites More sharing options...
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