Aethelfrith Posted May 7, 2015 Share Posted May 7, 2015 I'm interviewing for an enzymologist position at a pharma company. So the keys here are models of inhibition and assay design. Although I have some experience setting up kinetics assays, it was in a different context and with little/no support or criticism from anyone in my company. My questions are: When would one choose an endpoint assay over a continuous/real time one? In a kinase assay, what are some ADvantages of measuring ADP over the phosphorylated substrate? When would you choose a linearized plot (eg Lineweaver Burke, Eadie Hofstee) over a non linear plot? How would dose-response data on a semi-log vs. linear scale look? Thanks in advance. Link to comment Share on other sites More sharing options...
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