kl2owen Posted February 10, 2015 Share Posted February 10, 2015 I was in the lab yesterday making RIPA buffer with the flu and wasn't thinking straight.....I was weighing out my solids into the same beaker: sodium fluoride, SDS and sodium dioxycholate however I had rinsed my beaker first, so the bottom was wet...which was bad news for me as when I added the sodium diocholate last the reaction released a nasty vapour and the sodium dicholate turned yellow. I'm pretty sure a little hydrofluoric acid vapour was produced in this reaction but my stand-alone chemistry years are long behind me. Can anyone enlighten me as to what reaction would have occurred? Link to comment Share on other sites More sharing options...
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