biomart Posted February 7, 2015 Share Posted February 7, 2015 In the lab I am working, THP-1 Cells differentiated by PMA to Macrophage cell just for 24 hour. That is: we add PMA to THP-1 Cells and after 24 hour we change the media and without any delay we also treat the cells by our compound of interest to see changes in the cell signalling. I have read in some papers that I should let the cells to be differentiated for 72hr before doing any next step. what is you idea? Link to comment Share on other sites More sharing options...
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